Time (min) | Flow (ml/min) | A% | B% | 0 | 1ml/min | 65 | 35 | 15 | 65 | 35 | 20 | 55 | 45 | 26 | 55 | 45 | |
Column: Amethyst C18-P (5um, 4.6×150mm, 120Å SN:09100918716)
phase: A: 戊烷磺酸鈉1.5g+磷酸二氫銨3.5g+水950ml,用磷酸調(diào)pH=3.0,用水稀釋至1000mL
B:甲醇
Flow rate: 1.0mL/min Column Temp: 25℃ Wavelength: 287nm
Injection Volume: 10ul Pressures: 170.5~171.5Bar
Instrument:Agilent1200 自動進樣